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Development of normal mice from oocytes injected with freeze-dried spermatozoa

Nature Biotechnology volume 16, pages 639–641 (1998)Cite this article

Abstract

Freeze-dried mouse spermatozoa are all motionless and dead in the conventional sense. When injected into oocytes, however, their nuclei can support normal embryonic development even after three month preservation in a dried state. Although the freeze-drying protocol reported here will need further improvement, the results suggest that it may be possible to store the male genomes at room temperature.

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Authors and Affiliations

  1. Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu, HI

    Teruhiko Wakayama & Ryuzo Yanagimachi

  2. Department of Veterinary Anatomy, Faculty of Agriculture, University of Tokyo, Bunkyo-ku, Tokyo, 113, Japan

    Teruhiko Wakayama

Authors
  1. Teruhiko Wakayama
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  2. Ryuzo Yanagimachi
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Correspondence to Ryuzo Yanagimachi.

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Wakayama, T., Yanagimachi, R. Development of normal mice from oocytes injected with freeze-dried spermatozoa. Nat Biotechnol 16, 639–641 (1998). https://doi.org/10.1038/nbt0798-639

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  • DOI: https://doi.org/10.1038/nbt0798-639

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