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Complement recruitment using bispecific diabodies

Nature Biotechnology volume 15, pages 629–631 (1997)Cite this article

Abstract

We describe the engineering of antibody fragments produced in bacteria for recruitment of complement effector functions. From a phage display repertoire we isolated human antibody fragments directed against complement C1q, and linked these to lysozyme-specific antibody fragments, creating bispecific antibodies (diabodies). One diabody was able to recruit C1q, resulting in efficient lysis of lysozyme-coated sheep erythrocytes, and also induced rosette-formation of erythrocytes with human monocytes and phagocytosis after phorbol ester stimulation. These diabodies may have therapeutic applications requiring the activation of complement.

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Author notes

  1. Roland E. Kontermann

    Present address: Institut für Molekularbiologieund Tumor Forschung, Universität Marburg, Emil-Mannkopff-Str. 2, 35037, Marburg, Germany

  2. Mark G. Wing

    Present address: University of Cambridge Neurology Unit, Addenbrooke's Hospital, Cambridge, CB22QQ, UK

  3. Roland E. Kontermann: Corresponding author (e-mail: rek@imt.uni-marburg.de).

Authors and Affiliations

  1. MRC Centre for Protein Engineering,

    Roland E. Kontermann & Greg Winter

  2. MRC Molecular Immunopathology Unit,

    Mark G. Wing

  3. MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK

    Greg Winter

Authors
  1. Roland E. Kontermann
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  2. Mark G. Wing
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  3. Greg Winter
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Kontermann, R., Wing, M. & Winter, G. Complement recruitment using bispecific diabodies. Nat Biotechnol 15, 629–631 (1997). https://doi.org/10.1038/nbt0797-629

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  • DOI: https://doi.org/10.1038/nbt0797-629

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