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Genetic Engineering of Rice for Resistance to Sheath Blight

Bio/Technology volume 13, pages 686–691 (1995)Cite this article

Abstract

A 1.1 kb rice genomic DNA fragment, containing a chitinase gene under the control of the CaMV 35S promoter, was cloned into the rice transformation vector pGL2. After transformation of Indica rice protoplasts in the presence of polyethyleneglycol, plants were regenerated. The presence of the chimeric chitinase gene in T0 and T1 transgenic rice plants was detected by Southern blot analysis. Western blot analysis of transgenic plants and their progeny revealed the presence of two proteins with apparent molecular weights of 30 and 35 kDa that reacted with the chitinase antibody. Progeny from the chitinase-positive plants were tested for their resistance to the sheath blight pathogen, Rhizoctonia solani. The degree of resistance displayed by the transgenic plants to this pathogen correlated with the level of chitinase expression.

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Author information

Authors and Affiliations

  1. Department of Biochemistry, Kansas State University, Manhattan, Kansas, 66506

    Willie Lin, C.S. Anuratha & S. Muthukrishnan

  2. International Rice Research Institute, P.O. Box 933, 1099, Manila, The Philippines

    Karabi Datta & Swapan K. Datta

  3. ETH-Zurich, Institute of Plant Sciences, Zurich, CH-8092, Switzerland

    Karabi Datta, Ingo Potrykus & Swapan K. Datta

Authors
  1. Willie Lin
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  2. C.S. Anuratha
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  3. Karabi Datta
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  4. Ingo Potrykus
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  5. S. Muthukrishnan
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  6. Swapan K. Datta
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Corresponding author

Correspondence to S. Muthukrishnan.

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Lin, W., Anuratha, C., Datta, K. et al. Genetic Engineering of Rice for Resistance to Sheath Blight. Nat Biotechnol 13, 686–691 (1995). https://doi.org/10.1038/nbt0795-686

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