Research Paper | Published:

Antibody Production in Baculovirus-Infected Insect Cells

Bio/Technology volume 8, pages 651654 (1990) | Download Citation

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Abstract

We have employed the baculovirus expression system for the production of a mouse monoclonal IgG antibody directed against lipoprotein I of Pseudomonas aeruginosa. Both light and heavy chain cDNAs were introduced into the baculovirus genome in a single step of homologous recombination. Insect cells that were infected with the recombinant virus stably secreted antigen-binding and glycosylated antibody molecules capable of binding the complement component C1q.

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Author information

Author notes

    • Horst Domdey

    Corresponding author.

Affiliations

  1. Laboratorium für molekulare Biologie-Genzentrum-der Ludwig-Maximilians-Universität München, D-8033 Martinsried, FRG.

    • Jasper zu Putlitz
    • , Matthias Marget
    •  & Horst Domdey
  2. Present address: Department of Molecular Biology, Massachusetts General Hospital, Boston, MA 02114, USA.

    • William L. Kubasek
  3. Present address: Institut für allgemeine und experimentelle Pathologie, Universität Wien, A-1090 Wien, Austria.

    • Michael Duchêne
  4. Chirurgische Universitütsklinik, Chirurgische Forschung, D-7800 Freiburg, FRG.

    • Bernd-Ulrich von Specht

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DOI

https://doi.org/10.1038/nbt0790-651

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