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Antibody Production in Baculovirus-Infected Insect Cells

Bio/Technology volume 8, pages 651–654 (1990)Cite this article

Abstract

We have employed the baculovirus expression system for the production of a mouse monoclonal IgG antibody directed against lipoprotein I of Pseudomonas aeruginosa. Both light and heavy chain cDNAs were introduced into the baculovirus genome in a single step of homologous recombination. Insect cells that were infected with the recombinant virus stably secreted antigen-binding and glycosylated antibody molecules capable of binding the complement component C1q.

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Author notes

  1. William L. Kubasek

    Present address: Department of Molecular Biology, Massachusetts General Hospital, Boston, MA, 02114, USA

  2. Michael Duchêne

    Present address: Institut für allgemeine und experimentelle Pathologie, Universität Wien, A-1090, Wien, Austria

  3. Horst Domdey: Corresponding author.

Authors and Affiliations

  1. Laboratorium für molekulare Biologie-Genzentrum-der Ludwig-Maximilians-Universität München, D-8033, Martinsried, FRG

    Jasper zu Putlitz, Matthias Marget & Horst Domdey

  2. Chirurgische Universitütsklinik, Chirurgische Forschung, D-7800, Freiburg, FRG

    Bernd-Ulrich von Specht

Authors
  1. Jasper zu Putlitz
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  2. William L. Kubasek
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  4. Matthias Marget
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  5. Bernd-Ulrich von Specht
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  6. Horst Domdey
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zu Putlitz, J., Kubasek, W., Duchêne, M. et al. Antibody Production in Baculovirus-Infected Insect Cells. Nat Biotechnol 8, 651–654 (1990). https://doi.org/10.1038/nbt0790-651

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