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Gene Transfer into Loblolly Pine by Agrobacterium tumefaciens

Bio/Technology volume 4, pages 647–649 (1986)Cite this article

Abstract

We have demonstrated expression of bacterial genes transferred into cells of loblolly pine (Pinus taeda L.) by Agrobacterium tumefaciens. Whereas previous surveys found pines resistant to Agrobacterium, we found two wild type strains that produce galls. Callus proliferated from these galls synthesized specific opines. These results provide strong evidence for transfer and expression of bacterial genes in pine and extend the potential of genetic engineering to the world's most important genus for fiber production.

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Authors and Affiliations

  1. Institute of Forest Genetics and the Pacific Southwest Forest and Range Experiment Station, USDA Forest Service, P.O. Box 245, Berkeley, CA, 94701

    Ronald Sederoff

  2. Department of Forestry, North Carolina State University, Raleigh, NC, 27695

    Anne-Marie Stomp

  3. Department of Botany, North Carolina State University, Raleigh, NC, 27695

    W. Scott Chilton

  4. Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, 97331

    Larry W. Moore

Authors
  1. Ronald Sederoff
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  2. Anne-Marie Stomp
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  3. W. Scott Chilton
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  4. Larry W. Moore
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Sederoff, R., Stomp, AM., Chilton, W. et al. Gene Transfer into Loblolly Pine by Agrobacterium tumefaciens. Nat Biotechnol 4, 647–649 (1986). https://doi.org/10.1038/nbt0786-647

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