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Cell Culture Derived Recombinant HBsAg is Highly Immunogenic and Protects Chimpanzees from Infection with Hepatitis B Virus

Bio/Technology volume 4, pages 630–636 (1986)Cite this article

Abstract

Recombinant hepatitis B surface antigen (rHBsAg) particles, secreted from Chinese hamster ovary (CHO) cells stably transfected with the S gene of HBV, are indistinguishable antigenically from HBsAg particles derived from the plasma of patients chronically infected with hepatitis B virus (HBV). The recombinant vaccine containing CHO cell derived rHBsAg is more immunogenic in chimpanzees than previously reported vaccines of either plasma derived HBsAg or yeast derived rHBsAg based on the kinetics of antibody production and the maximum titers elicited. In addition, chimpanzees vaccinated with CHO cell–culture derived vaccine have a strong cellular immune response to rHBsAg as measured by cell proliferation assays. Animals vaccinated with cell culture derived rHBsAg (ad subtype) are protected from infection with both the ad and ay subtypes of HBV.

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Authors and Affiliations

  1. Assay Development Department, Genentech, Inc., 460 Point San Bruno Boulevard, South San Francisco, CA, 94080

    Eric J. Patzer & Gerald R. Nakamura

  2. Recovery Process Research and Development, Genentech, Inc., 460 Point San Bruno Boulevard, South San Francisco, CA, 94080

    Robert D. Hershberg & Timothy J. Gregory

  3. Molecular Biology, Genentech, Inc., 460 Point San Bruno Boulevard, South San Francisco, CA, 94080

    Craig Crowley & Arthur D. Levinson

  4. Virology and Immunology Department Southwest Foundation for Biomedical Research, San Antonio, TX, 78284

    Jorg W. Eichberg

Authors
  1. Eric J. Patzer
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  2. Gerald R. Nakamura
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  3. Robert D. Hershberg
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  4. Timothy J. Gregory
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  5. Craig Crowley
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  6. Arthur D. Levinson
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  7. Jorg W. Eichberg
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Patzer, E., Nakamura, G., Hershberg, R. et al. Cell Culture Derived Recombinant HBsAg is Highly Immunogenic and Protects Chimpanzees from Infection with Hepatitis B Virus. Nat Biotechnol 4, 630–636 (1986). https://doi.org/10.1038/nbt0786-630

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