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Vectors for Transformation of Higher Plants

Bio/Technology volume 3, pages 637–642 (1985)Cite this article

Abstract

We have constructed several vectors for the cloning of foreign DNA into plant genomes. These vectors are based on the transformation system of Agrobacterium tumefaciens and incorporate a number of useful features for plant cell transformation. The prototype vector, pEND4K, contains the left and right T-DNA border sequences and a chimeric gene which confers kanamycin resistance on transformed plant cells. The vector also includes the following features that facilitate the bacterial manipulations: the bacteriophage lambda cos site for in vitro packaging of large inserts, alpha complementation for direct screening of cloned inserts, and the wide host range RK2 replication and transfer origins. The vectors have been demonstrated to efficiently transform plant cells by several criteria.

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Author notes

  1. Harry J. Klee

    Present address: Monsanto Company, St. Louis, MO

Authors and Affiliations

  1. Department of Microbiology and Immunology, University of Washington, Seattle, WA, 98195

    Martin F. Yanofsky & Eugene W. Nester

Authors
  1. Harry J. Klee
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  2. Martin F. Yanofsky
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  3. Eugene W. Nester
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Klee, H., Yanofsky, M. & Nester, E. Vectors for Transformation of Higher Plants. Nat Biotechnol 3, 637–642 (1985). https://doi.org/10.1038/nbt0785-637

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