The pros and cons of peptide-centric proteomics

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Recommendations on how best to exploit the strengths of peptide-centric proteomics and avoid its pitfalls.

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Figure 1: General approach used by peptide-centric MS technologies for the identification of proteins in complex mixtures.
Figure 2: Representation of sequence coverage for a protein identified using a peptide-centric approach.

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Acknowledgements

We thank R. Nelson, A. Yergey and I. Krull for their insightful and constructive comments on early drafts of this manuscript.

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Correspondence to Richard M Caprioli.

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The authors declare no competing financial interests.

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