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Herbicide Resistant Fertile Transgenic Wheat Plants Obtained by Microprojectile Bombardment of Regenerable Embryogenic Callus

Bio/Technology volume 10, pages 667–674 (1992)Cite this article

Abstract

We have obtained fertile transgenic wheat plants resistant to the broad spectrum herbicide Basta® (active ingredient phosphinothricin, PPT) by high velocity microprojectile bombardment. The plasmid pBARGUS was used to deliver the selectable bar gene into cells of Type C long–term regenerable embryogenic callus. Phosphinothricin acetyltransferase (PAT) enzyme activity encoded by the bar gene was demonstrated in four independent putative transformed callus lines selected on Basta® from two cultivars. Although somatic embryos and shoots were formed in each of the four lines, plants were recovered only from two. More than 100 green R0 plants were regenerated from the first callus line, of which 40 were grown to maturity. PAT activity was shown in each of the 28 R0 plants tested. Southern analyses confirmed the presence of the bar gene in all of the callus lines, and in each of the R0 and two of the four R1 plants tested. Transformed R0, R1 and R2 plants were resistant to topical applications of Basta®, and the bar gene segregated as a dominant Mendelian trait in R1 and R2 plants.

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Author notes

  1. Indra K. Vasil: Corresponding author.

Authors and Affiliations

  1. Laboratory of Plant Cell and Molecular Biology, University of Florida, 1253 Fifield Hall, Gainesville, FL, 32611

    Vimla Vasil, Ana M. Castillo & Indra K. Vasil

  2. Monsanto Company, 700 Chesterfield Village Parkway, St. Louis, MO, 63198

    Michael E. Fromm

Authors
  1. Vimla Vasil
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  2. Ana M. Castillo
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  3. Michael E. Fromm
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  4. Indra K. Vasil
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Vasil, V., Castillo, A., Fromm, M. et al. Herbicide Resistant Fertile Transgenic Wheat Plants Obtained by Microprojectile Bombardment of Regenerable Embryogenic Callus. Nat Biotechnol 10, 667–674 (1992). https://doi.org/10.1038/nbt0692-667

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