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Segregation of Organelle Traits Following Protoplast Fusion in Nicotiana

Bio/Technology volume 3, pages 555–560 (1985)Cite this article

Abstract

Protoplasts from plants with different organelle-types were fused in order to monitor the fate of mixed cytoplasms in plants regenerated from fused protoplasts. Protoplasts of Nicotiana tabacum (tobacco) were fused with two experimental lines containing N. tabacum nucleus but containing the cytoplasm (chloroplasts and mitochondria) of either N. repanda or N. suaveolens. Fused cells were microisolated so that the plants regenerated could be traced to a single fusion product with mixed cytoplasms. In these fusion products the chloroplasts appeared to segregate randomly while mitochondria segregated toward male sterile-type. While most regenerated plants segregated by maturity some clones segregated for chloroplast type in the R1 generation implying the presence of two chloroplast types in individual cells of the parental clone. Data from these fusions gives us a better understanding of how organelles behave following protoplast fusion.

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Authors and Affiliations

  1. DNA Plant Technology Corporation, 2611 Branch Pike, Cinnaminson, NJ, 08077

    Christopher E. Flick, Stephanie A. Kut, Yuri Y. Gleba & David A. Evans

  2. Institute of Botany, Repina 2, Kiev, 252601, USSR

    Janis E. Bravo

Authors
  1. Christopher E. Flick
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  2. Stephanie A. Kut
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  3. Janis E. Bravo
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  4. Yuri Y. Gleba
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  5. David A. Evans
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Flick, C., Kut, S., Bravo, J. et al. Segregation of Organelle Traits Following Protoplast Fusion in Nicotiana. Nat Biotechnol 3, 555–560 (1985). https://doi.org/10.1038/nbt0685-555

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