Directed gene knockout in a vertebrate has been achieved using zinc-finger nucleases.
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Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat
BMC Immunology Open Access 08 November 2012
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References
Amsterdam, A. & Hopkins, N. Trends Genet. 22, 473–478 (2006).
Meng, X. et al. Nat. Biotechnol. 26, 695–701 (2008).
Doyon, Y. et al. Nat. Biotechnol. 26, 702–708 (2008).
Porteus, M.H. & Baltimore, D. Science 300, 763 (2003).
Bibikova, M., Beumer, K., Trautman, J.K. & Carroll, D. Science 300, 764 (2003).
Wu, J., Kandavelou, K. & Chandrasegaran, S. Cell. Mol. Life Sci. 64, 2933–2944 (2007).
Santiago, Y. et al. Proc. Natl. Acad. Sci. USA 105, 5809–5814 (2008).
Bibikova, M., Golic, M., Golic, K.G. & Carroll, D. Genetics 161, 1169–1175 (2002).
Urnov, F.D. et al. Nature 435, 646–651 (2005).
Mandell, J.G. & Barbas, C.F. Nucleic Acids Res. 34, W516–523 (2006).
Carroll, D., Morton, J.J., Beumer, K.J. & Segal, D.J. Nat. Protocols 1, 1329–1341 (2006).
Ramirez, C.L. et al. Nat. Methods 5, 374–375 (2008).
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Woods, I., Schier, A. Targeted mutagenesis in zebrafish. Nat Biotechnol 26, 650–651 (2008). https://doi.org/10.1038/nbt0608-650
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DOI: https://doi.org/10.1038/nbt0608-650
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