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Next-generation sequencing-by-hybridization

A new hybridization-based technology offers advantages in sequencing genomes for which a reference genome exists.

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Figure 1: Shotgun sequencing-by-hybridization (shotgun-SBH).


  1. Pihlak, A. et al. Nat. Biotechnol. 26, 676–684 (2008).

    Article  CAS  Google Scholar 

  2. Southern, E. United Kingdom Patent Application GB8810400 (1988).

  3. Drmanac, R., Petrovic, N., Glisin, V. & Crkvenjakov, R. Genomics 4, 114–128 (1989).

    Article  CAS  Google Scholar 

  4. Fire, A. & Xu, S.Q. Proc. Natl. Acad. Sci. USA 92, 4641–4645 (1995).

    Article  CAS  Google Scholar 

  5. Lizardi, P.M. et al. Nat. Genet. 19, 225–232 (1998).

    Article  CAS  Google Scholar 

  6. Koshkin, A.A. et al. Tetrahedron 54, 3607–3630 (1998).

    Article  CAS  Google Scholar 

  7. Porreca, G.J. et al. Nat. Methods 4, 931–936 (2007).

    Article  CAS  Google Scholar 

  8. Hodges, E. et al. Nat. Genet. 39, 1522–1527 (2007).

    Article  CAS  Google Scholar 

  9. Harris, T.D. et al. Science 320, 106–109 (2008).

    Article  CAS  Google Scholar 

  10. Jett, J.H. et al. J. Biomol. Struct. Dyn. 7, 301–309 (1989).

    Article  CAS  Google Scholar 

Download references

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Lizardi, P. Next-generation sequencing-by-hybridization. Nat Biotechnol 26, 649–650 (2008).

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