Nat. Biotechnol. 22, 589–594 (2004)
Technical note: Recent experiments with other 2A-linked constructs have suggested that cleavage efficiency can be influenced by the protein NH2-terminal to the 2A peptide. In some instances, we have found that cleavage efficiency can be improved by placing a Gly-Ser-Gly linker between NH2-terminal protein and the 2A peptide.
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Szymczak, A., Workman, C., Wang, Y. et al. Addendum: Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide–based retroviral vector. Nat Biotechnol 22, 760 (2004). https://doi.org/10.1038/nbt0604-760d
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DOI: https://doi.org/10.1038/nbt0604-760d
