Plants offer many advantages over bacteria as agents for bioremediation; however, they typically lack the degradative capabilities of specially selected bacterial strains. Transgenic plants expressing microbial degradative enzymes could combine the advantages of both systems. To investigate this possibility in the context of bioremediation of explosive residues, we generated transgenic tobacco plants expressing pentaerythritol tetranitrate reductase, an enzyme derived from an explosive-degrading bacterium that enables degradation of nitrate ester and nitroaromatic explosives. Seeds from transgenic plants were able to germinate and grow in the presence of 1 mM glycerol trinitrate (GTN) or 0.05 mM trinitrotoluene, at concentrations that inhibited germination and growth of wild-type seeds. Transgenic seedlings grown in liquid medium with 1 mM GTN showed more rapid and complete denitration of GTN than wild-type seedlings. This example suggests that transgenic plants expressing microbial degradative genes may provide a generally applicable strategy for bioremediation of organic pollutants in soil.
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Hoeppel, R.E. & Hinchee, R.E. in Hazardous Waste Site Soil Remediation: Theory and Application of Innovative Technologies (eds Wilson, D.J. & Clarke, A.N.) 311–431 (Marcel Dekker, New York, 1994).
Shannon, M.J.R. & Unterman, R. Evaluating bioremediation: distinguishing fact from fiction. Annu. Rev. Microbiol. 47, 715–738 (1993).
Cunningham, S.D. & Ow, D.W. Promises and prospects of phytoremediation. Plant Physiol. 110, 715–719 (1996).
Raskin, I. Plant genetic engineering may help with environmental cleanup. Proc. Natl. Acad. Sci. USA 93, 3164– 3166 (1996).
Rugh, C.L., Senecoff, J.F., Meagher, R.B. & Merkle, S.A. Development of transgenic yellow poplar for mercury phytoremediation. Nat. Biotechnol. 16, 925–928 (1998).
Binks, P.R., French, C.E., Nicklin, S. & Bruce, N.C. Degradation of pentaerythritol tetranitrate by Enterobacter cloacae PB2. Appl. Environ. Microbiol. 62, 1214– 1219 (1996).
French, C.E., Nicklin, S. & Bruce, N.C. Sequence and properties of pentaerythritol tetranitrate reductase from Enterobacter cloacae PB2. J. Bacteriol. 178, 6623–6627 (1996).
French, C.E., Nicklin, S. & Bruce, N.C. Aerobic degradation of 2,4,6-trinitrotoluene by Enterobacter cloacae PB2 and by pentaerythritol tetranitrate reductase. Appl. Environ. Microbiol. 64, 2864– 2868 (1998).
Goel, A., Kumar, G., Payne, G.F. & Dube, S.K. Plant cell biodegradation of a xenobiotic nitrate ester, nitroglycerin. Nat. Biotechnol. 15, 174–177 (1997).
Taylor, I.W., Ioannides, C. & Parke, D.V. Organic nitrate reductase: reassessment of its subcellular localization and tissue distribution and its relationship to the glutathione transferases. Int. J. Biochem. 21, 67– 71 (1989).
Hughes, J.B., Shanks, J., Vanderford, M., Lauritzen, J. & Bhadra, R. Transformation of TNT by aquatic plants and plant tissue cultures. Environ. Sci. Technol. 31, 266–271 (1997).
Guerineau, F., Lucy, A. & Mullineaux, P. Effect of two consensus sequences preceding the translation initiator codon on gene expression in plant protoplasts. Plant Mol. Biol. 18, 815–818 (1992).
Gleave, A.P. A versatile binary vector system with a T-DNA organizational structure conducive to efficient integration of cloned DNA into the plant genome. Plant Mol. Biol. 20, 1203–1207 (1992).
Ogawa, N. et al. Purification and characterization of glutathione-independent denitration enzyme of organic nitrate esters in rabbit hepatic cytosol. Biol. Pharm. Bull. 18, 1352–1355 (1995).
Schnoor, J.L., Licht, L.A., McCutcheon, S.C., Wolfe, N.L. & Carreira, L.H. Phytoremediation of organic and nutrient contaminants. Environ. Sci. Technol. 29, 318A–323A (1995).
Sambrook, J., Fritsch, E.F. & Maniatis, T. Molecular Cloning: A Laboratory Manual 2nd edn. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1989).
Horsch, R.B. et al. A simple and general method of transferring genes to plants. Science 227, 1229–1231 (1985).
This work was funded by a grant from the Defence Evaluation and Research Agency.
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French, C., Rosser, S., Davies, G. et al. Biodegradation of explosives by transgenic plants expressing pentaerythritol tetranitrate reductase. Nat Biotechnol 17, 491–494 (1999). https://doi.org/10.1038/8673
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