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Inhibition of a starch-granule–bound protein leads to modified starch and repression of cold sweetening

Nature Biotechnology volume 16, pages 473–477 (1998)Cite this article

Abstract

We have cloned a gene involved in starch metabolism that was identified by the ability of its product to bind to potato starch granules. Reduction in the protein level of transgenic potatoes leads to a reduction in the phosphate content of the starch. The complementary result is obtained when the protein is expressed in Escherichia coli, as this leads to an increased phosphate content of the glycogen. It is possible that this protein is responsible for the incorporation of phosphate into starch-like glucans, a process that is not understood at the biochemical level. The reduced phosphate content in potato starch has some secondary effects on its degradability, as the respective plants show a starch excess phenotype in leaves and a reduction in cold-sweetening in tubers.

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Authors and Affiliations

  1. Institut für Genbiologische Forschung Berlin GmbH, Ihnestr. 63, 14195, Berlin, Germany

    Ruth Lorberth, Lothar Willmitzer & Jens Kossmann

  2. Max-Planck-Institut für molekulare Pflanzenphysiologie, Karl-Liebknecht-Str. 24-25, 14476, Golm, Germany

    Ruth Lorberth, Lothar Willmitzer & Jens Kossmann

  3. Institut für Biochemie und molekulare Physiologic, Universität Potsdam, Maulbeerallee 2, 14469, Potsdam, Germany

    Ruth Lorberth & Gerhard Ritte

Authors
  1. Ruth Lorberth
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  2. Gerhard Ritte
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  3. Lothar Willmitzer
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  4. Jens Kossmann
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Correspondence to Jens Kossmann.

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Lorberth, R., Ritte, G., Willmitzer, L. et al. Inhibition of a starch-granule–bound protein leads to modified starch and repression of cold sweetening. Nat Biotechnol 16, 473–477 (1998). https://doi.org/10.1038/nbt0598-473

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