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A System for Production of Commercial Quantities of Human Lactoferrin: A Broad Spectrum Natural Antibiotic

Bio/Technologyvolume 13pages498503 (1995) | Download Citation

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Abstract

We previously reported the production of limited quantities of biologically active recombinant human lactoferrin in the filamentous fungus, Aspergillus oryzae. In the present study, we report a modification of this production system combined with a classical strain improvement program that has enabled production of levels of recombinant human lactoferrin in excess of 2 g/l. The protein was expressed in Aspergillus awamori as a glucoamylase fusion polypeptide which was secreted into the growth medium and processed to mature human lactoferrin by an endogenous KEX-2 peptidase. The recombinant protein retains full biological activity in terms of its ability to bind iron and human enterocyte receptors. Furthermore, the recombinant protein functions as a potent broad spectrum antimicrobial protein.

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Author notes

  1. Orla M. Conneely: Corresponding author.

Affiliations

  1. Department of Cell Biology, Baylor College of Medicine, Houston, TX, 77030

    • Pauline P. Ward
    • , Grainne A. Cunningham
    • , Xiaodong Zhou
    •  & Orla M. Conneely
  2. PANLABS, Inc., 11804 North Creek Parkway South, Bothell, WA, 98011-8805

    • Christopher S. Piddington
  3. Agennix, Inc., 7505 Fannin St., Suite 510, Houston, TX, 77054

    • Roger D. Wyatt

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https://doi.org/10.1038/nbt0595-498

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