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Production of Functional Human Hemoglobin in Transgenic Swine

Bio/Technology volume 10, pages 557–559 (1992)Cite this article

Abstract

A construct containing the locus control region (LCR) from the human β globin locus together with two copies of the human α1 gene and a single copy of the human βA gene was used to obtain three transgenic pigs. The transgenic pigs are healthy, not anemic, and grow at a rate comparable to non–transgenic littermates. All animals expressed the human genes. However, α globin was consistently expressed at higher levels than β globin. Isolation of the human hemoglobin from both porcine hemoglobin and other non–hemoglobin proteins was accomplished by ion exchange chromatography. The purified porcine derived human hemoglobin exhibited an oxygen affinity similar to that of human derived human hemoglobin.

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Author notes

  1. Carl A. Pinkert

    Present address: University of Alabama at Birmingham, 1870 University Blvd., Birmingham, AL, 35294-0019

  2. John S. Logan: Corresponding author.

Authors and Affiliations

  1. DNX, Inc., 303B College Road East, Princeton, NJ, 08540

    Mark E. Swanson, Michael J. Martin, J. Kevin O'Donnell, Kathy Hoover, William Lago, Victoria Huntress, Cynthia T. Parsons, Stephen Pilder & John S. Logan

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  1. Mark E. Swanson
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  2. Michael J. Martin
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Swanson, M., Martin, M., O'Donnell, J. et al. Production of Functional Human Hemoglobin in Transgenic Swine. Nat Biotechnol 10, 557–559 (1992). https://doi.org/10.1038/nbt0592-557

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