Abstract
We report here what we believe is the first example of the encapsulation of hybridoma cells within a synthetic polymer by a simple gelation with dissolved cations in water, and at room temperature. Two lines of hybridoma cells were encapsulated within calcium cross-linked polyphospha-zene gel microbeads without affecting their viability or their capability to produce antibodies. Interaction of these gel beads with the positively-charged poly-electrolyte, poly(L-lysine), of 102-kD molecular weight, produced a semipermeable membrane that was capable of retaining the cell-secreted antibodies inside the beads. Cell density increased 3.5-fold within 13 days concomitant with a 6.4-fold increase in antibody production. These synthetic membranes have the potential to aid in protein recovery schemes.
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Bañó, M., Cohen, S., Visscher, K. et al. A Novel Synthetic Method for Hybridoma Cell Encapsulation. Nat Biotechnol 9, 468–471 (1991). https://doi.org/10.1038/nbt0591-468
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DOI: https://doi.org/10.1038/nbt0591-468
