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High–Frequency Transformation of Penicillium Chrysogenum



We report high–frequency transformation of penicillin–producing strains of Penicillium chrysogenum using plasmid vectors carrying the pyr4 gene of Neurospora crassa. Transformation to stable uracil independence is associated with acquisition of transforming DNA in high molecular forms that are not simple multimers of the vector. A transformant strain showed a fivefold higher orotidine–5′–monophosphate decarboxylase activity (coded by the pyr4 gene) than the wild type strain, indicating that the genes carried in the transforming vector are efficiently expressed in Penicillium.

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