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Cloning of the Gamma–Subunit Methane Monooxygenase from Methylococcus Capsulatus


Methane monooxygenase (MMO) catalyses the oxidation of a variety of organic compounds in addition to the oxidation of methane to methanol in methanotrophs. In order to complement biochemical and physiological studies on MMO from Methylococcus capsulatus, we have initiated molecular biological studies on this enzyme system. We obtained the amino acid sequence of the N–terminus of the γ–subunit of MMO, and used oligonucleotide mixed probes corresponding to this sequence to locate in a gene library a recombinant plasmid which contains the γ–subunit gene.

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