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Expression of Active Human Immunodeficiency Virus Reverse Transcriptase in Saccharomyces Cerevisiae

Bio/Technologyvolume 5pages486489 (1987) | Download Citation

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Abstract

The reverse transcriptase (RT) domain of the human immunodeficiency virus (HIV) pol gene was expressed in yeast. Purification and analysis of the recombinant protein indicated that yeast post–translational processing of RT was similar, if not identical, to that which had previously been observed in HIV infected lymphoid cells. In addition, recombinant RT was enzymatically active and could be inhibited by several anti–retroviral agents. These studies indicate that HIV RT expressed in yeast will be useful for the in vitro analysis of further potential inhibitors of this important enzyme.

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Author notes

  1. Philip J. Barr: Corresponding author.

Affiliations

  1. Chiron Corporation, 4560 Horton Street, Emeryville, CA, 94608

    • Philip J. Barr
    • , Michael D. Power
    • , Chun Ting Lee-Ng
    • , Helen L. Gibson
    •  & Paul A. Luciw

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https://doi.org/10.1038/nbt0587-486