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Customized signaling with reconfigurable protein scaffolds

Nature Biotechnology volume 26, pages 526–528 (2008)Cite this article

Engineering protein scaffolds creates signaling networks with novel properties.

The emerging field of synthetic biology aims to design sophisticated biological systems that exploit diverse mechanisms for regulating information flow. New functions have been implemented using engineered transcriptional and translational networks, but little progress has been achieved in constructing protein-protein networks with complex connectivities. A recent report in Science by Lim and colleagues1 addresses this challenge with a general approach for controlling signaling by protein scaffolds, as demonstrated by engineering of the pheromone response of the mitogen-activated protein kinase (MAPK) pathway in yeast.

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Figure 1: Examples of signaling topologies using the engineered scaffold protein.

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Authors and Affiliations

  1. Patrick Guye and Ron Weiss are in the Departments of Molecular Biology and Electrical Engineering, Olden Street, Princeton University, Princeton, New Jersey 08544, USA. rweiss@princeton.edu,

    Patrick Guye & Ron Weiss

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  1. Patrick Guye
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  2. Ron Weiss
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Guye, P., Weiss, R. Customized signaling with reconfigurable protein scaffolds. Nat Biotechnol 26, 526–528 (2008). https://doi.org/10.1038/nbt0508-526

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  • DOI: https://doi.org/10.1038/nbt0508-526

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