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Full-length antibodies on display

Nature Biotechnology volume 25, pages 537–538 (2007)Cite this article

Display of IgG libraries on bacteria promises to streamline antibody engineering.

The transformation of antibodies over the last decade from research tools into the most successful class of targeted therapeutics could not have occurred without key advances in antibody engineering in microorganisms1. But the complex structure of full-length antibodies has necessitated a cumbersome 'divide and conquer' approach—antibodies are first dissected into fragments that can be engineered in microorganisms and then the optimized fragments are stitched back into the full-length form for production in mammalian cells. In this issue, Georgiou and colleagues2 present an approach that circumvents these limitations by allowing libraries of full-length antibodies to be screened in Escherichia coli.

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Figure 1: Development of E-clonal antibody display.

Katie Ris

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Authors and Affiliations

  1. Sachdev S. Sidhu is in the Department of Protein Engineering, Genentech, Inc., 1 DNA Way, S. San Francisco, California 94080, USA. sidhu@gene.com,

    Sachdev S Sidhu

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  1. Sachdev S Sidhu
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Sidhu, S. Full-length antibodies on display. Nat Biotechnol 25, 537–538 (2007). https://doi.org/10.1038/nbt0507-537

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