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Bringing picomolar protein detection into proximity

Combining target recognition by two aptamers, enzymatic ligation, and PCR, the proximity ligation method enables the detection of minute amounts of proteins.

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Figure 1: The proximity ligation assay can be 1,000-fold more sensitive than a conventional ELISA.

© Bob Crimi

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Famulok, M. Bringing picomolar protein detection into proximity. Nat Biotechnol 20, 448–449 (2002). https://doi.org/10.1038/nbt0502-448

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