Selection of high-affinity phage antibodies from phage display libraries

Abstract

Successful screening of phage display libraries will lead to more efficient, inexpensive antibody production.

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Figure 1: Procedure and experimental results for selection of displayed antibodies.
Figure 2: Monitoring insert size in phagemid populations.
Figure 3: Performance of anti-NH3 phages isolated by high-stringency elution.

References

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Acknowledgements

We are grateful to Greg Winter and collaborators for providing the Griffin.1 library and to Rosella Franconi and Eugenio Benvenuto for helpful discussions.

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Correspondence to Francesca Quattrocchio.

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Bruin, R., Spelt, K., Mol, J. et al. Selection of high-affinity phage antibodies from phage display libraries. Nat Biotechnol 17, 397–399 (1999). https://doi.org/10.1038/7959

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