Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • In the Laboratory
  • Published:

Selection of high-affinity phage antibodies from phage display libraries

Nature Biotechnology volume 17, pages 397–399 (1999)Cite this article

Abstract

Successful screening of phage display libraries will lead to more efficient, inexpensive antibody production.

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Procedure and experimental results for selection of displayed antibodies.
Figure 2: Monitoring insert size in phagemid populations.
Figure 3: Performance of anti-NH3 phages isolated by high-stringency elution.

References

  1. Kay, B.K. et al., eds. Phage display of peptides and proteins: a laboratory manual (Academic Press, San Diego, CA, 1996).

    Google Scholar 

  2. Griffiths, A.D. et al. Curr. Opin. Biotechnol. 9, 102–108 (1998).

    Article  CAS  Google Scholar 

  3. Griffiths, A.D. et al. EMBO J. 13, 3245–3260 (1994).

    Article  CAS  Google Scholar 

  4. Mol, J. et al. Trends Plant Sci. 3, 212–217 (1998).

    Article  Google Scholar 

  5. Souer, E. et al. Cell 85, 159–170 (1996).

    Article  CAS  Google Scholar 

  6. Aida, M. et al. Plant Cell 9, 841–857 (1997).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We are grateful to Greg Winter and collaborators for providing the Griffin.1 library and to Rosella Franconi and Eugenio Benvenuto for helpful discussions.

Author information

Authors and Affiliations

  1. Department of developmental genetics, Institute for Molecular Biological Sciences, Vrije Universiteit, BioCentrum Amsterdam, de Boelelaan 1087, Amsterdam, 1081 HV, The Netherlands

    Robert de Bruin, Kees Spelt, Joseph Mol, Ronald Koes & Francesca Quattrocchio

Authors
  1. Robert de Bruin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kees Spelt
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Joseph Mol
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ronald Koes
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Francesca Quattrocchio
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Francesca Quattrocchio.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bruin, R., Spelt, K., Mol, J. et al. Selection of high-affinity phage antibodies from phage display libraries. Nat Biotechnol 17, 397–399 (1999). https://doi.org/10.1038/7959

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/7959

This article is cited by

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing