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Simultaneous Amplification and Detection of Specific DNA Sequences

Bio/Technology volume 10, pages 413–417 (1992)Cite this article

Abstract

We have enhanced the polymerase chain reaction (PCR) such that specific DNA sequences can be detected without opening the reaction tube. This enhancement requires the addition of ethidium bromide (EtBr) to a PCR. Since the fluorescence of EtBr increases in the presence of double–stranded (ds) DNA an increase in fluorescence in such a PCR indicates a positive amplification, which can be easily monitored externally. In fact, amplification can be continuously monitored in order to follow its progress. The ability to simultaneously amplify specific DNA sequences and detect the product of the amplification both simplifies and improves PCR and may facilitate its automation and more widespread use in the clinic or in other situations requiring high sample through–put.

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Author notes

  1. Russell Higuchi: Corresponding author.

Authors and Affiliations

  1. Roche Molecular Systems, Inc., 1400 53rd St., Emeryville, CA, 94608

    Russell Higuchi, P. Sean Walsh & Robert Griffith

  2. Chiron Corporation, 1400 53rd St., Emeryville, CA, 94608

    Gavin Dollinger

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  1. Russell Higuchi
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  2. Gavin Dollinger
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  3. P. Sean Walsh
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  4. Robert Griffith
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Higuchi, R., Dollinger, G., Walsh, P. et al. Simultaneous Amplification and Detection of Specific DNA Sequences. Nat Biotechnol 10, 413–417 (1992). https://doi.org/10.1038/nbt0492-413

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  • DOI: https://doi.org/10.1038/nbt0492-413

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