We have developed nuclear magnetic resonance (NMR) flow imaging techniques to measure fluid flow in a cell-free hollow fiber bioreactor (HFBR). Using 1H NMR we track the motion of protons and obtain velocity distributions as a function of position and time. These measurements enable the visualization of flow patterns needed for module design and for establishing desired operating conditions. Uneven flow in the cell-containing region of an HFBR can result in concentration gradients and uneven cell distribution that may lead to reduced cell viability. Results from this non-invasive method could be used to design more efficient cell bioreac-tors or membrane separation devices.
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Hammer, B., Heath, C., Mirer, S. et al. Quantitative Flow Measurements in Bioreactors by Nuclear Magnetic Resonance Imaging. Nat Biotechnol 8, 327–330 (1990). https://doi.org/10.1038/nbt0490-327
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