Research Paper | Published:

Genetic Control Systems of Escherichia Coli Can Confer Inducible Expression of Cloned Genes in Coryneform Bacteria

Bio/Technology volume 6, pages 428430 (1988) | Download Citation

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Abstract

We have investigated the use of the Escherichia coli lac and trp operator–represser and λ phage PRPL operator–cI represser systems to control expression of transformed genes in an amino acid–producing coryneform bacterium, Brevibacterium lactofermentum. We report here that functional lac, trp and ,cI repressers are produced in B. lactofermentum from lacI, trpR and cI857 genes containing their original promoters. Transcription of a chloram–phenicol acetyl transferase (CAT) gene with tac, lac, trp, or PR PL control regions could be blocked by three heterogeneous repressors. Addition of inducers or shift of cultivation temperature triggered marked derepression of CAT expression. These repressor–operator complexes may be useful as artificial “on/off switches” in the regulation of gene expression in the coryneform bacteria.

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Author notes

    • Makoto Tsuchiya

    Corresponding author.

Affiliations

  1. Central Research Laboratories of Ajinomoto Co., Inc., 1-1 Suzuki-cho, Kawasaki-ku, Kawasaki 210, Japan.

    • Makoto Tsuchiya
    •  & Yasushi Morinaga

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DOI

https://doi.org/10.1038/nbt0488-428

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