Research Paper | Published:

Stable Expression of Aspercillus Awamori Glucoamylase in Distiller's Yeast

Bio/Technology volume 6, pages 417421 (1988) | Download Citation

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Abstract

A strain of distiller's yeast stably secreting high levels of Aspergillus awamori glucoamylase was constructed by introducing an expression cassette containing glucoamylase cDNA and a dominant selectable marker into the yeast chromosome. Glucoamylase expression levels were increased by modifying the yeast promoter and the sequences upstream of the start of translation. Fermentation studies with the improved strain showed that production of glucoamylase was constant for over 800 generations of continuous culture. This strain utilizes about 95% of the carbohydrates in soluble starch at 25% (w/v) substrate concentration and produces high yields of ethanol.

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Author information

Author notes

    • Duane Inlow
    •  & Arie Ben-Bassat

    Fermentation Research and Development, Cetus Corporation, 1400 53rd St., Emeryville, CA 94608.

    • Michael A. Innis

    Corresponding author.

Affiliations

  1. Departments of Microbial Genetics, Cetus Corporation, 1400 53rd St., Emeryville, CA 94608.

    • Georgette E. Cole
    • , Peter C. McCabe
    • , Duane Inlow
    • , David H. Gelfand
    • , Arie Ben-Bassat
    •  & Michael A. Innis

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DOI

https://doi.org/10.1038/nbt0488-417

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