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Synthesis and Assembly in Yeast of Hepatitis B Surface Antigen Particles Containing the Polyalbumin Receptor


Yeast cells are genetically engineered to synthesize and assemble hepatitis B surface antigen particles containing 55 additional amino acids encoded by the hepatitis B virus preS gene. These particles are composed of a 28,000 dalton protein and efficiently bind polymerized human albumin. Antibodies to the polyalbumin receptor epitope of this particle may interfere with the postulated polyalbumin-mediated binding of HBV to hepatocytes and may therefore be particularly efficient in preventing the disease.

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Valenzuela, P., Coit, D. & Kuo, C. Synthesis and Assembly in Yeast of Hepatitis B Surface Antigen Particles Containing the Polyalbumin Receptor. Nat Biotechnol 3, 317–320 (1985).

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