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Experimental Methods for On–Line Mass Spectrometry in Fermentation Technology*

Bio/Technology volume 1, pages 181188 (1983) | Download Citation

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Abstract

It is shown here how mass spectrometry (MS) can be used for on–line data acquisition in fermentation. MS was applied in this work to analyze gas and liquid phases. Gas phase analysis allows fast and accurate measurement of all gases of interest (O2, N2, CO2, Ar, He etc.). Liquid phase analysis is possible with a steam sterilizable membrane probe and permits direct analysis of dissolved gases (O2, CO2, N2) and various volatiles. Automatic switching between gas inlet and membrane probe and the data reduction was accomplished by desk computer. Continuous yeast fermentation was monitored over long periods to study long–term stability, which seems to be adequate for fermentation processes.

*Parts of this work were presented at the 2nd European Congress Biotechnology in Eastbourne, U.K., 1981, and at the 9th International Mass Spectrometry Conference in Vienna, A, 1982.

**Symbols used in this paper appear preceding the reference section.

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References

  1. 1.

    and 1981. On-line mass spectrometry evaluation and implementation, Dyn. Mass Spectrometry 6: 155–166.

  2. 2.

    , , , and 1981. Quadrupole mass spectrometry in process monitoring and control, Dyn. Mass Spectrometry 6: 167–180.

  3. 3.

    1967. Der Beitrag der Massenspektrometrie zur Steuerung und Kontrolle industrieller Fertigungsprozesse. Z. Instr. 75: 190–195.

  4. 4.

    , and 1981. The use of time-of-flight mass spectrometry (TOFMS) in process monitoring and control. Dyn. Mass Spectrometry. 6: 181–192.

  5. 5.

    , , , , and 1981. Use of an automated mass spectrometer for an underground coal gasification field test. Dyn. Mass Spectrometry 6: 195–211.

  6. 6.

    , , and 1949. A mass spectrometer for continuous gas analysis. Rev. Scient. Instrum. 20: 333–336.

  7. 7.

    and 1972. p. 601–622. Clinical uses of mass spectrometry. In: Biochemical applications of Mass Spectrometry. Waller G. R. (ed.), Wiley Interscience, New York.

  8. 8.

    , , and 1970. On-line analysis of reactor products by mass spectrometry. Chem. Eng. (London) N244: CE413–CE418.

  9. 9.

    and 1978. Real time control of industrial processes by application of a quadrupole M.S. computer system. Adv. Mass Spectrometry 7B: 1062–1068.

  10. 10.

    1980. Instrumentation and control in fermentation: The application of computer controlled mass spectrometry. 5th Intern. Ferm. Symp., London, Canada (abstr).

  11. 11.

    , , , , , and 1978. Capillary system for continuous introducing of volatile liquids into analytical mass spectrometers and its applications. Adv. Mass Spectrometry 7B: 858–864.

  12. 12.

    , , , and 1981. Continuous monitoring of fermentation outlet gas using a computer coupled MS. Biotechnol. Letters 2: 409–414.

  13. 13.

    1981. Ein mobiles Massenspektrometersystem zur Erfassung umweltbelastender Schadstoffe. Hochschule der Bundeswehr, Hamburg. (Ph.D. thesis).

  14. 14.

    1981. A quadrupole system for atmospheric pollution monitoring. Dyn. Mass Spectrometry 6: 212–219.

  15. 15.

    and 1981. Membranes in Separations. Wiley and Sons (Wiley Interscience), New York.

  16. 16.

    and 1979. The use in physiology of a rapidly responding mass-spectrometer sensor. Recent Dev. Mass Spectrom. Biochem. Med. 6: 483–487.

  17. 17.

    , , and 1966. Blood gases; continuous in vivo recording of partial pressures by mass spectrography. Science. 153: 885–887.

  18. 18.

    , , , , and 1970. A non thrombogenic diffusion membrane for continuous in vivo measurement of blood gases by mass spectrometry. J. Appl. Physiol. 28: 375–377.

  19. 19.

    , , , , and 1979. The development of a flexible mass spectrometer catheter. Recent Dev. Mass Spectrom. Biochem. Med. 5: 463–480.

  20. 20.

    , , and 1975. Application of mass spectrometry to the measurement of dissolved gases and volatile substances in fermentation. Europe. J. Appl. Microbiol. Biotechnol. 1: 323–325.

  21. 21.

    and 1980. Continuous mass spectrometric measurement of dissolved H2, O2 and CO2 during chemolitho-autotrophic growth of Alcaligenes eutrophus strain H 16. Europ. J. Appl. Microbiol. Biotechnol. 11: 17–22.

  22. 22.

    , , , and 1981. p. 393–398. Direct monitoring of a fermentation in a computer-mass spectrometer-fermentor system. In: Advances in Biotechnology. 1: M. MooYoung, C. W. Robinson, and C. Vezina (ed). Pergamon Press, Toronto.

  23. 23.

    , , , , and 1980. Continuous monitoring, by mass spectrometry, of H2 production and recycling in Rhodopseudomonas capsulata. J. Bacteriol. 143: 628–636.

  24. 24.

    , , and 1976. p. 168–183. Mass spectrometric determination of oxygen kinetics in biochemical systems. In: Measurement of Oxygen. Proc. Interdisc. Symp., H. Degn and R. Brook (Ed.) Elsevier, Amsterdam.

  25. 25.

    , , , and 1982. Process analysis of the acetone-butanol fermentation by quadrupole mass spectrometry. Biotechnol. Letters 4: 557–562.

  26. 26.

    , , , and 1976. Biochemical assay by immobilized enzymes and a mass spectrometer. p. 207–225. Biochem. Biophys. Acta 438: 296–303.

  27. 27.

    1977. Possible biomedical applications of the volatile enzyme product method. In: Biomedical Applications of Immobilized Enzymes and Proteins. 2: T.M.S. Chang (ed.) Plenum Press.

  28. 28.

    and 1979. Use of variable pH interface to a mass spectrometer for the measurement of dissolved volatile compounds. Rev. Sci. Instrum. 50: 478–481.

  29. 29.

    , , , and 1980. Temporarily immobilized microorganisms: rapid measurements using a mass spectrometer. Biotechnol. Letters 2: 133–137.

  30. 30.

    1969. Katabolit-Repression und der Sprossungszyklus von Saccharomyces cerevisiae. Diss. ETH no. 4279, Zurich.

  31. 31.

    Krasnobajev, V. Givaudan, Dubendorf, CH (private communication).

  32. 32.

    , , , and Influence of oxygen on the growth of Saccharomyces cerevisiae in continuous culture. Biotechnol. Bioeng. (in press).

  33. 33.

    , , , and 1982. Modelling of sustained oscillations observed in continuous culture of Saccharomyces cerevisiae. 1st Workshop on Modelling and Control of Biotechnical Processes. IFAC, Helsinki.

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  1. Technisch-chemisches Laboratorium ETH, CH-8092 Zurich, Switzerland

    • E. Heinzle
    • , K. Furukawa
    • , I. J. Dunn
    •  & J. R. Bourne

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DOI

https://doi.org/10.1038/nbt0483-181

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