It is shown here how mass spectrometry (MS) can be used for on–line data acquisition in fermentation. MS was applied in this work to analyze gas and liquid phases. Gas phase analysis allows fast and accurate measurement of all gases of interest (O2, N2, CO2, Ar, He etc.). Liquid phase analysis is possible with a steam sterilizable membrane probe and permits direct analysis of dissolved gases (O2, CO2, N2) and various volatiles. Automatic switching between gas inlet and membrane probe and the data reduction was accomplished by desk computer. Continuous yeast fermentation was monitored over long periods to study long–term stability, which seems to be adequate for fermentation processes.
*Parts of this work were presented at the 2nd European Congress Biotechnology in Eastbourne, U.K., 1981, and at the 9th International Mass Spectrometry Conference in Vienna, A, 1982.
**Symbols used in this paper appear preceding the reference section.
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Heinzle, E., Furukawa, K., Dunn, I. et al. Experimental Methods for On–Line Mass Spectrometry in Fermentation Technology*. Nat Biotechnol 1, 181–188 (1983). https://doi.org/10.1038/nbt0483-181
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