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Locking in on the human methylome

Two groups have combined padlock probes and massively parallel sequencing to characterize cytosine methylation in targeted regions of the human genome.

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Figure 1: High-throughput analysis of genomic methylation using padlock probes and bisulfite-treated DNA3,4 or methylation-sensitive cut counting4.

References

  1. Cokus, S.J. et al. Nature 452, 215–219 (2008).

    CAS  Article  Google Scholar 

  2. Lister, R. et al. Cell 133, 523–536 (2008).

    CAS  Article  Google Scholar 

  3. Deng, J. et al. Nat. Biotechnol. 27, 353–360 (2009).

    CAS  Article  Google Scholar 

  4. Ball, M.P. et al. Nat. Biotechnol. 27, 361–368 (2009).

    CAS  Article  Google Scholar 

  5. Meissner, A. et al. Nature 454, 766–770 (2008).

    CAS  Article  Google Scholar 

  6. Nilsson, M. et al. Science 265, 2085–2088 (1994).

    CAS  Article  Google Scholar 

  7. Porreca, G.J. et al. Nat. Methods 4, 931–936 (2007).

    CAS  Article  Google Scholar 

  8. Down, T.A. et al. Nat. Biotechnol. 26, 779–785 (2008).

    CAS  Article  Google Scholar 

  9. Jones, P.A. Trends Genet. 15, 34–37 (1999).

    CAS  Article  Google Scholar 

  10. Rauch, T.A. et al. Proc. Natl. Acad. Sci. USA 106, 671–678 (2009).

    CAS  Article  Google Scholar 

  11. Birney, E. et al. Nature 447, 799–816 (2007).

    CAS  Article  Google Scholar 

  12. Eckhardt, F. et al. Nat. Genet. 38, 1378–1385 (2006).

    CAS  Article  Google Scholar 

Download references

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Competing interests

P.W.L. is a Scientific Advisory Board member and consultant for Epigenomics, AG, which has a commercial interest in DNA methylation diagnostics.

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Berman, B., Weisenberger, D. & Laird, P. Locking in on the human methylome. Nat Biotechnol 27, 341–342 (2009). https://doi.org/10.1038/nbt0409-341

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  • DOI: https://doi.org/10.1038/nbt0409-341

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