Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

Finding needles in haystacks

Probes that reversibly recognize oligohistidine sequences engineered into proteins can provide insights into the structure and location of proteins in living cells.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Determining ligand-receptor interactions on membrane proteins with nickel nitrilotriacetate (Ni2+-NTA) probes.

Bob Crimi

References

  1. Guignet, E.G., Hovius, R. & Vogel, H. Nat. Biotechnol. 22, 440–444 (2004).

    Article  CAS  Google Scholar 

  2. Karlin, A. & Akabas, M.H. Methods Enzymol. 293, 123–145 (1998).

    Article  CAS  Google Scholar 

  3. Javitch, J.A., Shi, L. & Liapakis, G. Methods Enzymol. 343, 137–156 (2002).

    Article  Google Scholar 

  4. Tsien, R.Y. Annu. Rev. Biochem. 67, 509–544 (1998).

    Article  CAS  Google Scholar 

  5. Griffin, B.A., Adams, S.R. & Tsien, R.Y. Science 281, 269–272 (1998).

    Article  CAS  Google Scholar 

  6. Adams, S.R. et al. J. Am. Chem. Soc. 124, 6063–6076 (2002).

    Article  CAS  Google Scholar 

  7. Nakanishi, J. et al. Anal. Chem. 73, 2920–2928 (2001).

    Article  CAS  Google Scholar 

  8. Rice, M.C., Czymmek, K. & Kmiec, E.B. Nat. Biotechnol. 19, 321–326 (2001).

    Article  CAS  Google Scholar 

  9. Tour, O., Meijer, R.M., Zacharias, D.A., Adams, S.R. & Tsien, R.Y. Nat. Biotechnol. 21, 1505–1508 (2003).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Javitch, J. Finding needles in haystacks. Nat Biotechnol 22, 394–396 (2004). https://doi.org/10.1038/nbt0404-394

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/nbt0404-394

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing