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Large-scale functional analysis using peptide or protein arrays

Nature Biotechnology volume 18, pages 393–397 (2000)Cite this article

Abstract

The array format for analyzing peptide and protein function offers an attractive experimental alternative to traditional library screens. Powerful new approaches have recently been described, ranging from synthetic peptide arrays to whole proteins expressed in living cells. Comprehensive sets of purified peptides and proteins permit high-throughput screening for discrete biochemical properties, whereas formats involving living cells facilitate large-scale genetic screening for novel biological activities. In the past year, three major genome-scale studies using yeast as a model organism have investigated different aspects of protein function, including biochemical activities, gene disruption phenotypes, and protein–protein interactions. Such studies show that protein arrays can be used to examine in parallel the functions of thousands of proteins previously known only by their DNA sequence.

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Figure 1: Screening for function using libraries and arrays.
Figure 2: Arrays may be nonliving or living.

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Acknowledgements

We thank Michael DeVit, Andrew Emili, Stanley Fields, Stephen McCraith, Eric Phizicky, Chandra Tucker, and Peter Uetz for comments on the manuscript, and Deborah Diamond for helpful suggestions. This work was supported by NIH grants GM54415 and RR11823 and a grant from the Merck Genome Research Institute. A.Q.E. is a research associate of the Howard Hughes Medical Institute.

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Authors and Affiliations

  1. Departments of Genetics and Medicine, University of Washington, Seattle, 98195, WA

    Alia Qureshi Emili & Gerard Cagney

  2. Howard Hughes Medical Institute, University of Washington, Seattle, 98195, WA

    Alia Qureshi Emili

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  1. Alia Qureshi Emili
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Correspondence to Gerard Cagney.

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Emili, A., Cagney, G. Large-scale functional analysis using peptide or protein arrays. Nat Biotechnol 18, 393–397 (2000). https://doi.org/10.1038/74442

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