Research Article | Published:

Biomagnetic isolation of antigen-specific CD8+ T cells usable in immunotherapy

Nature Biotechnology volume 16, pages 281285 (1998) | Download Citation

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Abstract

Isolating antigen-specific T lymphocytes is hampered by the low frequency of the cells and the low affinity between T-cell receptors (TCR) and antigen. We describe the isolation and purification of antigen-specific CD8+ T lymphocytes from mixed T-cell populations. Magnetic beads coated with major histocompatibility complex class I molecules loaded with specific peptide were used as a substrate for T-cell capture. Low-frequency T cells, as well as T cells with TCR of low affinity for the antigen were captured on the beads. Following isolation and expansion, recovered cells specifically killed target cells in vitro, and displayed antiviral effect in vivo.

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Author information

Author notes

    • Alain T. Luxembourg

    e-mail: aluxembourg@liai.org

Affiliations

  1. R. W. Johnson Pharmaceutical Research Institute, San Diego, CA 92121.

    • Anders B. Brunmark
    • , Per A. Peterson
    •  & Michael R. Jackson
  2. Current address: La Jolla Institute for Allergy and Immunology, San Diego, CA 92121.

    • Alain T. Luxembourg
  3. The Scripps Research Institute, La Jolla, CA 92037.

    • Persephone Borrow
  4. Current address: The Edward Jenner Institute for Vaccine Research, Compton, Newbury, Berkshire, RG20 7NN, UK.

    • Persephone Borrow
  5. Current address: The Scripps Research Institute, La Jolla, CA 92037.

    • Luc Teyton

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DOI

https://doi.org/10.1038/nbt0398-281

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