Research Article | Published:

Peptide mimics of a tumor antigen induce functional cytotoxic T cells

Nature Biotechnology volume 16, pages 276280 (1998) | Download Citation

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Abstract

The ability to mimic peptide/peptide and/or peptide/carbohydrate structures may be important in generating cross-reactive antibodies for autoimmune and other diseases. We show that the peptide sequence DAHWESWL can mimic the conformation of the unrelated MUC1 peptide SAPDTRPAP(G). Mice immunized with mannan-MUC1-peptides make cytotoxic T lymphocytes (CTLs) and are protected from MUC1+ tumors. We show that the same specific anti-MUCI responses can be produced by immunizing with the DAHWESWL peptide; furthermore, specific tumor protection is obtained in a manner similar to that with MUC1 immunization. The DAHWESWL peptide immunization leads to CTLs that recognize H2Dd and H2Ld but not H2b or human leukocyte antigens–group A (HLA-A)*0201 presented MUC1 peptides. However, mutation of the DAHWESWL peptide to a more HLA-A*0201–compatible structure with appropriate anchors (DLHWASWV), leads to the production of CTLs in HLA-A*0201 mice.

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Author information

Affiliations

  1. The Austin Research Institute, Studley Rd., Heidelberg, 3084, Victoria, Australia.

    • Vasso Apostolopoulos
    • , Shari A. Lofthouse
    • , Violeta Popovski
    • , Mauro S. Sandrin
    •  & lan F.C. McKenzie
  2. John Curtin School of Medical Research, Department of Human Genetics, PO Box 334, Canberra ACT 2601, Australia.

    • Gareth Chelvanayagam

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Correspondence to lan F.C. McKenzie.

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DOI

https://doi.org/10.1038/nbt0398-276

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