Research Article | Published:

Peptide mimics of the CTLA4-binding domain stimulate T-cell proliferation

Nature Biotechnology volume 16, pages 267270 (1998) | Download Citation

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Abstract

Phage library clones selected by a conformational epitope-recognizing and inhibitory monoclonal antibody may display moieties that mimic a receptor/ligand-like three-dimensional structure. This pseudoreceptor/ligand should be able to bind to natural ligand/receptor molecules. We tested this idea using anti-T cell costimulatory molecule antibodies and successfully isolated phage clones with costimulatory effects on T-cell proliferation. This strategy facilitates the designing of regulatory peptide molecules in the absence of precise information about the structure-function relationships in receptor/ligand interactions.

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Affiliations

  1. Department of Bioengineering, Faculty of Engineering, Kagoshima University, Korimoto, Kagoshima 890, Japan.

    • Takahiro Fukumoto
    • , Naohiko Torigoe
    • , Shinichi Kawabata
    • , Masaaki Murakami
    • , Yuji Ito
    •  & Kazuhisa Sugimura
  2. Institute of lmmunological Science, Hokkaido University, Kitaku, Sapporo 060, Japan.

    • Toshimitu Uede
  3. Department of Neurosurgery, School of Medicine, Kumamoto University, Kumamoto 860, Japan.

    • Tohru Nishi

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Correspondence to Kazuhisa Sugimura.

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DOI

https://doi.org/10.1038/nbt0398-267

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