Abstract
Phage library clones selected by a conformational epitope-recognizing and inhibitory monoclonal antibody may display moieties that mimic a receptor/ligand-like three-dimensional structure. This pseudoreceptor/ligand should be able to bind to natural ligand/receptor molecules. We tested this idea using anti-T cell costimulatory molecule antibodies and successfully isolated phage clones with costimulatory effects on T-cell proliferation. This strategy facilitates the designing of regulatory peptide molecules in the absence of precise information about the structure-function relationships in receptor/ligand interactions.
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Fukumoto, T., Torigoe, N., Kawabata, S. et al. Peptide mimics of the CTLA4-binding domain stimulate T-cell proliferation. Nat Biotechnol 16, 267–270 (1998). https://doi.org/10.1038/nbt0398-267
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DOI: https://doi.org/10.1038/nbt0398-267
