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Expansion of Human Bone Marrow Progenitor Cells in a High Cell Density Continuous Perfusion System

Bio/Technologyvolume 11pages368372 (1993) | Download Citation



We describe here a continuous perfusion bioreactor system that enables a population of unselected human mononuclear bone marrow cells obtained from adult donors to expand up to 20 to 25-fold over a two-week period. Colony-forming units of granulocyte-macrophage (CFU-GM) progenitor cells expand 10 to 30-fold. These expansions depend on the gas phase oxygen concentration, the seeding density and time of cell harvest. Under operating conditions that allow for good cell proliferation, 3 to 4 million mononuclear cells can be obtained per square centimeter, with 0.5 to 0.8% being progenitor cells. Autologous human sera supported cell expansion as efficiently as animal sera. Increasing the size of the perfusion system to produce a clinically meaningful number of CFU-GMs could have important applications in bone marrow transplantation therapies.

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Author notes

    • Se-Hwan Paek

    Present address: Central Research Institute, Lucky Ltd., Daejon, Rep. of Korea

    • Gyun-Min Lee

    Present address: Dept. of Biotechnology, Korean Advanced Institute of Science and Technology, Daejon, Rep. of Korea

  1. Bernhard O. Palsson: Corresponding author.


  1. Cellular Biotechnology Laboratory, Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, 48109

    • Bernhard O. Palsson
    • , Se-Hwan Paek
    •  & Gyun-Min Lee
  2. Aastrom Biosciences, Inc., Ann Arbor, MI, 48109

    • Richard M. Schwartz
    •  & Mahshid Palsson
  3. Hematology/Oncology Section, Dept. of Internal Medicine, University of Michigan, Ann Arbor, MI, 48109

    • Samuel Silver
    •  & Stephen G. Emerson


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