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DnaK-Mediated Alterations in Human Growth Hormone Protein Inclusion Bodies

Bio/Technology volume 10, pages 301–304 (1992)Cite this article

Abstract

Protein overproduction in microbes frequently results in protein misfolding and aggregation though the molecular basis for this process is unclear. The HSP70 chaperonin, DnaK, was identified as an important factor controlling heterologous protein aggregation in Escherichia coli. Co-overproduction of DnaK significantly reduced human growth hormone (HGH) protein inclusion body formation and the extent of HGH aggregation.

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Author notes

  1. Paul Blum

    Present address: School of Biological Sciences, University of Nebraska, Lincoln, NE, 68588-0118

  2. Abdul Matin: Corresponding author.

Authors and Affiliations

  1. Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, 94305-5402

    Paul Blum & Abdul Matin

  2. Genentech, Inc., 460 Point San Bruno Blvd., South San Francisco, CA, 94080

    Mark Velligan & Norman Lin

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  1. Paul Blum
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  2. Mark Velligan
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  3. Norman Lin
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  4. Abdul Matin
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Blum, P., Velligan, M., Lin, N. et al. DnaK-Mediated Alterations in Human Growth Hormone Protein Inclusion Bodies. Nat Biotechnol 10, 301–304 (1992). https://doi.org/10.1038/nbt0392-301

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