Research Paper | Published:

Continuous Fermentation with Fluidized Slurries of Immobilized Microorganisms

Bio/Technology volume 3, pages 247251 (1985) | Download Citation

Subjects

Abstract

The development of a continuous fermentation system with immobilized microorganisms for manufacturing biochemicals is described. Data for Saccharomyces cerevisiae producing ethanol is used as an example of reactor kinetics. A mini-bioreactor was used to obtain the kinetic data, which is useful in designing pilot plant and production scale reactors. Electron micrographs illustrate how the yeast cells populate the IMC beads and escape into the fermentation liquor. A method using cascaded fluidized beds with individual recirculation loops for oxygenation and fluidization is described.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1.

    1980. Immobilized microbes, p. 105–121. In: Annual Reports on Fermentation Processes. Vol. 4. D. Perlman (ed.), Academic Press, New York.

  2. 2.

    1977. Immobilized cells, p. 205–233. In: Annual Reports on Fermentation Processes, Vol. 1. D. Perlman (ed.), Academic Press, New York.

  3. 3.

    1978. Immobilized cells, p. 91–123. In: Annual Reports on Fermentation Processes, Vol. 2. D. Perlman (ed.), Academic Press, New York.

  4. 4.

    and 1978. Immobilized microbial cells. Process Biochemistry 13: 14–23.

  5. 5.

    and 1950. Experiments with the chemostat on spontaneous mutations of bacteria. Proc. Nat. Acad. of Sci. 36: 708–719.

  6. 6.

    1979. Immobilized microbial cells with polyacrylamide gel and carrageenan and their industrial applications, p. 187–202. In: Immobilized Microbial Cells. K. Venkatasubramanian (ed.), ACS Symp. Series 106, Washington, DC.

  7. 7.

    , and 1983. Chemical engineering analysis of immobilized cell systems. Applied Biochemistry and Bioengineering 4: 311–349.

  8. 8.

    and , 1983. Process Engineering Considerations in the Development of Immobilized Living Cell Systems, p. 133–144. In: Immobilized Cells and Organdies, Vol. 2. B. Mattiason (ed.), CRC Press, Boca Raton, Florida.

  9. 9.

    , , , , , and 1983. Entrapment of animal cells for the production of monoclonal antibodies and other biomolecules. Nature 302: 629–630.

  10. 10.

    and 1980. Preparation of immobilized animal cells. FEBS Letters 118: 145–150.

  11. 11.

    and 1982. Kinetic parameter estimation for reaction systems of immobilized cells and enzymes. Presented at 75th Annual AIChE Meeting in Los Angeles, CA, November 14–18.

  12. 12.

    and 1978. Process design and economic studies of alternative fermentation methods for the production of ethanol. Biotech and Bioeng. 20: 1421–1444.

Download references

Author information

Author notes

    • K. Venkatasubramanian

    Department of Chemical and Biochemical Engineering, Rutgers Univ., Piscataway, NJ

Affiliations

  1. Verax Corporation, Hanover, NH 03755

    • Subhash B. Karkare
    •  & Robert C. Dean Jr.

Authors

  1. Search for Subhash B. Karkare in:

  2. Search for Robert C. Dean in:

  3. Search for K. Venkatasubramanian in:

About this article

Publication history

Received

Accepted

Published

DOI

https://doi.org/10.1038/nbt0385-247

Further reading