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Response to Do amniotic fluid–derived stem cells differentiate into neurons in vitro?

Nature Biotechnology volume 26, pages 270–271 (2008)Cite this article

Cesar C Santos, Mark E Furth, Evan Y Snyder, Paolo De Coppi, Shay Soker & Anthony Atala respond:

We thank Toselli et al. for their comments on the neuronal differentiation of AFS cells. The goal of our report in Nature Biotechnology was to demonstrate that stem cells isolated from amniotic fluid are capable of self-renewal and of differentiation toward lineage pathways corresponding to the three embryonic germ layers1. Accordingly, we provided evidence that multiple, independent isolates of human AFS cells “were able to differentiate along adipogenic, osteogenic, myogenic, endothelial, neurogenic and hepatic pathways.” Within this group, the early neurogenic pathway served as the example for the ability to yield cells derived in development from the ectoderm. We further used retroviral marking to conclude that the multipotentiality of AFS cells with respect to the three germ layers is a clonal property.

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Competing interests

A.A. assigned to Children's Hospital Boston a patent involved with this technology and Children's Hostpital licensed the patent to Plueon, Inc. A.A. serves as a member of the board of directors of Plureon, Inc.

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Response to Do amniotic fluid–derived stem cells differentiate into neurons in vitro?. Nat Biotechnol 26, 270–271 (2008). https://doi.org/10.1038/nbt0308-270

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