Research Paper | Published:

Growth Enhancement in Transgenic Atlantic Salmon by the Use of an “All Fish” Chimeric Growth Hormone Gene Construct

Bio/Technologyvolume 10pages176181 (1992) | Download Citation

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Abstract

We have developed an “all fish” growth hormone (GH) chimeric gene construct by using an antifreeze protein gene (AFP) promoter from ocean pout linked to a chinook salmon GH cDNA clone. After microinjection into fertilized, nonactivated Atlantic salmon eggs via the micropyle, transgenic Atlantic salmon were generated. The presence of the transgene was detected by polymerase chain reaction (PCR) using specific oligonucleotide primers. A number of these transgenic fish showed dramatic increases in their growth rate. At one year old, the average increase of the transgenic fish was 2 to 6 fold and the largest transgenic fish was 13 times that of the average non-transgenic control.

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Author notes

  1. Choy L. Hew: Corresponding author.

Affiliations

  1. Research Institute, The Hospital for Sick Children and Departments of Clinical Biochemistry and Biochemistry, University of Toronto, Toronto, Canada, M5G 1L5

    • Shao Jun Du
    • , Zhiyuan Gong
    •  & Choy L. Hew
  2. Ocean Sciences Centre, Memorial University of Newfoundland, St. John's, Newfoundland, Canada, A1C 557

    • Garth L. Fletcher
    • , Margaret A. Shears
    • , Madonna J. King
    •  & David R. Idler

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DOI

https://doi.org/10.1038/nbt0292-176

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