Research Paper | Published:

Construction of a 7-Aminocephalosporanic Acid (7ACA) Biosynthetic Operon and Direct Production of 7ACA in Acremonium Chrysogenum

Bio/Technology volume 9, pages 188191 (1991) | Download Citation

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Abstract

We have used cDNA encoding D-amino acid oxidase, and genomic DNA encoding cephalosporin acylase from Fusarium solani and Pseudomonas diminuta, respectively, to construct a novel hybrid 7-aminocephalosporanic acid (7ACA) biosynthetic operon under the control of regulatory elements from the alkaline protease gene of Acremonium chrysogenum. Transformants of A. chrysogenum BC2116, a high cephalosporin-producing strain, containing this operon, synthesized and secreted low levels of 7ACA. Although the amounts are not yet commercially significant, this represents the first microbial production of 7ACA and demonstrates the feasibility of introducing new biosynthetic capabilities into industrial microorganisms by combining fungal and bacterial genes.

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Author notes

    • Takao Isogai

    Corresponding author.

Affiliations

  1. Exploratory Research Laboratories, Fujisawa Pharmaceutical Co. Ltd, Tokodai, Tsukuba, Ibaraki 300-26, Japan.

    • Takao Isogai
    • , Masao Fukagawa
    • , Ichiro Aramori
    • , Morita Iwami
    • , Hitoshi Kojo
    •  & Takaharu Ono
  2. Research and Development Group, Fujisawa Pharmaceutical Co. Ltd, Kashima, Yodogawa-ku, Osaka 532, Japan.

    • Yoshio Ueda
    • , Masanobu Kohsaka
    •  & Hiroshi Imanaka

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DOI

https://doi.org/10.1038/nbt0291-188

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