Research Paper | Published:

Heritable Damage to Yeast Caused by Transformation

Bio/Technology volume 9, pages 179182 (1991) | Download Citation

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Abstract

The introduction of plasmid DNA into yeast by transformation or electroporation, but not by cytoduction, results in the induction of a slow growth phenotype. This phenotype is inherited as a dominant Mendelian trait, which is only exhibited in the absence of the native 2μ nuclear DNA plasmid of yeast. The use of recombinant DNA technology in yeast, therefore, does not necessarily manipulate the genome in a precise and completely defined way.

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Author notes

    • Stephen G. Oliver

    Corresponding author.

Affiliations

  1. Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology, PO Box 88, Manchester M60 1QD, UK.

    • Nadia Danhash
    • , David C. J. Gardner
    •  & Stephen G. Oliver

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DOI

https://doi.org/10.1038/nbt0291-179

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