Research Paper | Published:

Regulated Expression Allows High Level Production and Secretion of HIV-1 gp120 Envelope Glycoprotein in Drosophila Schneider Cells

Bio/Technologyvolume 9pages173177 (1991) | Download Citation

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Abstract

We have established a stable, continuous culture Drosophila Schneider 2 cell line that efficiently expresses a secreted, truncated form of the HIV envelope gp120 protein in a regulated manner. The Drosophila produced recombinant gp120 protein is highly glycosylated, is recognized by gp120-specific monoclonal antibodies, binds to the CD4 receptor and has the ability to inhibit syncytia formation between uninfected CD4+ cells and HIV infected cells. We conclude that this recombinant Drosophila envelope protein is an appropriate mimic of the authentic viral envelope protein. Thus, the Drosophila cell provides a continuous, stable culture system for the efficient expression of secreted forms of complex surface glycoproteins in quantities sufficient for detailed analyses.

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Author notes

  1. Jeffery S. Culp: Corresponding author.

Affiliations

  1. Departments of Protein Biochemistry and Gene Expression Sciences, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania, 19406

    • Jeffery S. Culp
    • , Hanne Johansen
    • , Brian Hellmig
    • , Jeffery Beck
    •  & Martin Rosenberg
  2. Department of Surgery, Duke University Medical Center, Durham, North Carolina, 27710

    • Thomas J. Matthews
  3. Department of Molecular and Cellular Biology, SmithKline Beecham Biologicals, B-1330, Rixensart, Belgium

    • Anne Delers

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https://doi.org/10.1038/nbt0291-173

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