Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Materials and Methods
  • Published:

Automated Laser-Fluorescence Sequencing

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

References

  1. Ansorge, W., Sproat, B.S., Stegemann, J. and Schwager, C. 1986. A non-radioactive automated method for DNA sequence determination. J. Biochem. Biophys. Meth. 13: 315.

    Article  CAS  Google Scholar 

  2. Ansorge, W., Sproat, B.S., Stegemann, J., Schwager, C. and Zenke, M. 1987. Automated DNA sequencing: ultrasensitive detection of fluorescent bands during electrophoresis. Nucl. Acids Res. 15: 4593.

    Article  CAS  Google Scholar 

  3. Hultman, T., Ståhl, S., Hornes, E. and Uhlén, M. 1989. Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support. Nucl. Acids Res. 17: 4937.

    Article  CAS  Google Scholar 

  4. Voss, H., Schwager, C., Wirkner, U., Sproat, B., Zimmerman, J., Rosenthal, A., Erfle, H., Stegemann, J. and Ansorge, W. 1989. Direct genomic fluorescent on-line sequencing and analysis using in vitro amplification of DNA. Nucl. Acids Res. 17: 2517.

    Article  CAS  Google Scholar 

  5. Sanger, F., Nicklen, S. and Coulson, A.R. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. 74: 5463.

    Article  CAS  Google Scholar 

  6. Maxam, A.M. and Gilbert, W. 1980. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 101: 499.

    Article  Google Scholar 

  7. Kristensen, T., Voss, H. and Ansorge, W. 1987. A simple and rapid preparation of M13 sequencing templates for manual and automated dideoxy sequencing. Nucl. Acids Res. 15: 5507.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Freeman, M., Baehler, C. & Spotts, S. Automated Laser-Fluorescence Sequencing. Nat Biotechnol 8, 147–148 (1990). https://doi.org/10.1038/nbt0290-147

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/nbt0290-147

This article is cited by

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing