Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Expression of the Escherichia coli Enterotoxin Subunit B Gene in Aspergillus nidulans Directed by the AMDS Promoter

Bio/Technology volume 7, pages 169–174 (1989)Cite this article

Abstract

We have constructed an expression vector for the filamentous fungus Aspergillus nidulans using the well characterized amdS promoter system, and tested its potential as an expression system for foreign proteins using the Escherichia coli heat labile enterotoxin subunit B (LTB) gene. Co–transformation with a selectable plasmid was used to introduce an LTB construct into A. nidulans and transformants containing multiple copies of the construct were identified. DNA and mRNA coding for LTB were detected in transformants, and mRNA levels were inducible by acetate via the amdI9 mutation in the expression vector. Mature LTB was detected in cellular extracts but not in the medium, and expression levels correlated with the number of LTB genes.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Get just this article for as long as you need it

$39.95

Learn more

Prices may be subject to local taxes which are calculated during checkout

References

  1. Cullen, D. and Leong, S. 1986. Recent advances in the molecular genetics of industrial filamentous fungi. Trends in Biotech. 4: 285–288.

    Article  CAS  Google Scholar 

  2. Upshall, A. 1986. Filamentous fungi in biotechnology. Bio/Techniques 4: 158–164.

    CAS  Google Scholar 

  3. Van Brunt, J. 1986. Fungi: the perfect hosts? Bio/Technology 4: 1057–1062

    Article  CAS  Google Scholar 

  4. Cullen, D., Gray, G.L., Wilson, L.J., Hayenga, K.J., Lamsa, M.H., Rey, M.W., Norton, S., and Berka, R.M. 1987. Controlled expression and secretion of bovine chymosin in Aspergillus nidulans . Bio/Technology 5:369–376.

    CAS  Google Scholar 

  5. Gwynne, D.I., Buxton, F.P., Williams, S.A., Garven, S., and Davies, R.W. 1987. Genetically engineered secretion of active human inter-feron and a bacterial endoglucanase from Aspergillus nidulans . Bio/ Technology 5:713–719.

    CAS  Google Scholar 

  6. Upshall, A., Kumar, A.A., Bailey, M.C., Parker, M.D., Favreau, M.A., Lewison, K.P., Joseph, M.L., Maraganore, J.M., and McKnight, G.L. 1987. Secretion of active human tissue plasminogen activator from the filamentous fungus Aspergillus nidulans . Bio/Technology 5:1301–1304.

    CAS  Google Scholar 

  7. Hynes, M.J. 1978. Multiple independent control mechanisms affecting the acetamidase of Aspergillus nidulans . Mol. Gen. Genet. 161 59–65.

    Article  CAS  PubMed  Google Scholar 

  8. Hynes, M.J., Corrick, C.M., and King, J.A. 1983. Isolation of genomic clones containing the amdS gene of Aspergillus nidulans and their use in the analysis of structural and regulatory mutations. Mol. Cell. Biol. 3:1430–1439.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Andrianopoulos, A. and Hynes, M.J. 1988. Cloning and analysis of the positively acting regulatory gene amdR from Aspergillus nidulans . Mol. Cell. Biol. 8:3532–3541.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Caddick, M.X., Arst, H.N., Taylor, L.H., Johnson, R.I., and Brown-lee, A.G. 1986. Cloning of the regulatory gene areA indicating nitrogen metabolite repression in Aspergillus nidulans . EMBO Jour. 5:1087–1090.

    Article  CAS  Google Scholar 

  11. Corrick, C.M., Twomey, A.P., and Hynes, M.J. 1987. The nucleotide sequence of the amdS gene of Aspergillus nidulans and the molecular characterization of 5′ mutations. Gene 53:63–71.

    Article  CAS  PubMed  Google Scholar 

  12. Levine, M.M., Kaper, J.B., Black, R.E., and Clements, M.L. 1983. New knowledge on pathogenesis of bacterial enteric infections as applied to vaccine development. Microbiological Reviews 47:510–550.

    CAS  PubMed  PubMed Central  Google Scholar 

  13. Dallas, W.S. and Falkow, S. 1980. Amino acid sequence homology between cholera toxin and Escherichia coli heat-labile toxin. Nature 288: 499–500.

    Article  CAS  PubMed  Google Scholar 

  14. De Aizpurua, H.J. and Russell-Jones, G.J. 1988. Oral vaccination: identification of classes of proteins that provoke an immune response upon oral feeding. J. Exp. Med. 167:440–451.

    Article  CAS  PubMed  Google Scholar 

  15. Hynes, M.J. 1975. A cis-dominant regulatory mutation affecting enzyme induction in the eukaryote Aspergillus nidulans . Nature 253:210–212.

    Article  CAS  PubMed  Google Scholar 

  16. Hynes, M.J. 1982. A cis-dominant mutation in Aspergillus nidulans affecting the expression of the amdS gene in the presence of mutations in the unlinked gene, amdA. Genetics 102:139–147.

    CAS  PubMed  PubMed Central  Google Scholar 

  17. Durrens, P., Green, P.M., Arst, H.N., and Scazzocchio, C. 1986. Heterologous insertion of transforming DNA and generation of new deletions associated with transformation in Aspergillus nidulans . Mol. Gen. Genet. 203:544–549.

    Article  CAS  PubMed  Google Scholar 

  18. Kelly, J.M. and Hynes, M.J. 1987. Multiple copies of the amdS gene of Aspergillus nidulans cause titration of trans-acting regulatory proteins. Curr. Genet. 11:21–31.

    Article  Google Scholar 

  19. Hynes, M.J. 1977. Induction of the acetamidase of Aspergillus nidulans by acetate metabolism. J. Bacteriol. 131:770–775.

    CAS  PubMed  PubMed Central  Google Scholar 

  20. Von Heijne, G. 1984. How signal sequences maintain cleavage specificity. J. Mol. Biol. 173:243–251.

    Article  CAS  PubMed  Google Scholar 

  21. Schatz, G. 1986. A common mechanism for different membrane systems. Nature 321:108–109.

    Article  CAS  PubMed  Google Scholar 

  22. Perlman, D. and Halvorson, H.O. 1983. A putative signal peptidase recognition site and sequence in eukaryotic and prokaryotic signal peptides. J. Mol. Biol. 167:391–409.

    Article  CAS  PubMed  Google Scholar 

  23. Rothman, J.E. 1987. Protein sorting by selective retention in the endoplasmic reticulum and golgi stack. Cell 50:521–522.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Cohen, B.L. 1973. The neutral and alkaline proteases of Aspergillus nidulans . J. Gen. Microbiol. 77:521–528.

    Article  CAS  PubMed  Google Scholar 

  25. Tilburn, J., Scazzocchio, C., Taylor, G.G., Zabicky-Zissman, J.H., Lockington, R.A., and Davies, R.W. 1983. Transformation by integration in Aspergillus nidulans . Gene 26:205–221.

    Article  CAS  PubMed  Google Scholar 

  26. Maniatis, T., Fritsch, E.F., and Sambrook, J. 1982. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.

  27. Southern, E.M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98:503–517.

    Article  CAS  PubMed  Google Scholar 

  28. Rigby, P.W.J., Dieckmann, M., Rhodes, C., and Berg, P. 1977. Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J. Mol. Biol. 113:237–251.

    Article  CAS  PubMed  Google Scholar 

  29. Glisin, V., Crkvenjakov, R., and Byus, C. 1974. Ribonucleic acid isolated by cesium chloride centrifugation. Biochemistry 13:2633–2637.

    Article  CAS  PubMed  Google Scholar 

  30. Lehrach, H., Diamond, D., Wozney, J.M., and Boedtker, H. 1977. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical re-examination. Biochemistry J. 16: 4743–4751.

    Article  CAS  Google Scholar 

  31. Thomas, P.S. 1980. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc. Natl. Acad. Sci. USA 77:5201–5205.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  32. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685.

    Article  CAS  PubMed  Google Scholar 

  33. Towbin, H., Staehelin, T., and Gordon, J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350–4354.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  34. Sack, D.A., Huda, S., Neogi, P.K.B., Daniel, R.R., and Spira, W.M. 1980. Microtiter ganglioside enzyme-linked immunosorbent assay for vibrio and Escherichia coli heat-labile enterotoxins and antitoxin. J. Clin. Microbiol. 11:35–40.

    CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Author notes

  1. Michael J. Hynes: Corresponding author.

Authors and Affiliations

  1. Department of Genetics, University of Melbourne, Parkville, Victoria, 3052, Australia

    Ian F. Turnbull & Michael J. Hynes

  2. Biotechnology Australia Pty. Ltd., 28 Barcoo St., East Roseville, N.S.W., 2069, Australia

    Keith Rand & Neil S. Willetts

Authors
  1. Ian F. Turnbull
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Keith Rand
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Neil S. Willetts
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Michael J. Hynes
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Turnbull, I., Rand, K., Willetts, N. et al. Expression of the Escherichia coli Enterotoxin Subunit B Gene in Aspergillus nidulans Directed by the AMDS Promoter. Nat Biotechnol 7, 169–174 (1989). https://doi.org/10.1038/nbt0289-169

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nbt0289-169

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing