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Cloning Systems in Amino Acid–Producing Corynebacteria

Bio/Technology volume 5, pages 137–146 (1987)Cite this article

Abstract

Members of the genera Corynebacterium and Brevibacterium are widely used in the production of amino acids and nucleotides, as well as in the bioconversion of steroids and in the cheese industry. In the last few years, cloning vectors have been developed in corynebacteria using replicons from endogenous plasmids and antibiotic resistance genes as selectable markers. Promoter–probe plasmids, high–expression vectors, transposon mutagenesis, and in vitro systems for RNA and protein synthesis are now being developed. The availability of cloned genes and transformation and transfection systems in corynebacteria will facilitate gene amplification studies, and the removal of bottlenecks in the amino acid biosynthetic pathways, thus leading to increased production of amino acids.

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  1. Departamento de Microbiología, Facultad de Biología, Universidad de León, Spain

    J. F. Martín, R. Santamaría, H. Sandoval, G. del Real, L. M. Mateos, J. A. Gil & A. Aguilar

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Martín, J., Santamaría, R., Sandoval, H. et al. Cloning Systems in Amino Acid–Producing Corynebacteria. Nat Biotechnol 5, 137–146 (1987). https://doi.org/10.1038/nbt0287-137

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